Coding

Part:BBa_K5233012:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-30)


MHETase(Amcut), a kind of tannase family protein that can degrade MHET


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1553
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1553
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1553
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1553
    Illegal AgeI site found at 109
    Illegal AgeI site found at 526
    Illegal AgeI site found at 1176
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The MHETase is used for degrading MHET to TPA and promoting the whole process of degradation of PET. Since it is also connected with Amcut siganl peptide, it can be secreted outside the bacteria to function.

Source

It comes from Ideonella sakaiensis 201-F6

References

Janatunaim RZ, Fibriani A. Construction and Cloning of Plastic-degrading Recombinant Enzymes (MHETase). Recent Pat Biotechnol. 2020;14(3):229-234. doi: 10.2174/1872208314666200311104541. PMID: 32160855.